PRODUCT
LONG®R³ IGF-I Cell Culture Supplement
200 times more potent and two times more stable in cell culture than insulin,
LONG® R3 IGF-I (LONG R3) is a recombinant analog of human insulin-like
growth factor-I (IGF-I) that has been specifically engineered for the
enhancement of cell culture performance.
HIGH PERFORMANCE
More Potent than Insulin
LONG® R3 IGF-I is a recombinant analog of human insulin-like growth factor-I (IGF-I) specifically engineered to enhance cell culture performance.
- More biologically potent in vitro than either insulin or native IGF-I
- Ideal for systems utilizing serum-free or low-level serum applications
- Increased cell density, higher viability and extended culture duration
Better science for better cell culture
LONG® technology consists of a fusion of the growth factor with a portion of growth hormone sequence.
The fusion improves growth factor production without imparting its biological activity.
A distinct biological advantage
All mammalian cells secrete IGF Binding Proteins (IGFBPs) which bind to and inhibit native IGF-I.
The substitution of an arginine for glutamic acid at position three in LONG® R3 IGF-I,
in conjunction with the 13 amino acid N-terminal extension peptide,
results in > 1000 fold reduced affinity for IGFBPs, enhancing its bio-availability and
effectiveness in comparison to native IGF-I.
Specificity is the key to increased productivity
Unlike insulin, LONG® R3 IGF-I specifically targets and activates the type I IGF Receptor (IGF-IR) resulting
in greater activation of downstream signaling cascades responsible for proliferation and inhibiting apoptosis.
Greater activation of these key pathways results in increased cell density,
higher viability and extended cell culture productivity.
To learn more about the use of LONG® R3 IGF-I in stem cell and cell therapy applications, please contact Repligen.
Optimizing Cell Culture Productivity
Cell culture supplements are essential for long-term growth and productivity of cell lines in serum-free media
formulations. Both insulin and LONG® R3 IGF-I, a more potent supplement that directly targets and
activates the IGF-I receptor, are employed by the biopharmaceutical industry today to support the growth of
recombinant cell lines.
This webinar will show how these two supplements, used separately and in combination,
impact viable cell density and IgG production of multiple CHO cell lines. It will demonstrate how a combination
of LONG® R3 IGF-I and insulin results in less protein supplementation and, therefore, less potential impact
on downstream processing.
Improved volumetric productivity
LONG® R3 IGF-I has been shown to improve volumetric productivity
in CHO cell culture by up to 62% over cell culture with
no supplementation and 40% over cell culture supplemented with insulin.
LONG® R3 IGF-I Cell Culture Supplement
LONG® R3 IGF-I is a critically important mitogenic growth factor often used in mammalian cell culture to increase yield significantly.
Such growth factors are fundamental supplements to chemically-defined media.
LONG® R3 IGF-I ELISA Kit
The only commercially available kit for the quantitation of LONG® R3 IGF-I, the kit is fast, accurate, and easy to use with both cell culture and drug substance samples.
The sandwich ELISA kit uses a biotinylated secondary antibody and Streptavidin-HRP for detection.
- Limited reactivity to native or recombinant IGF-1
- No reactivity to insulin
- Ready-to-use ELISA plate allows the assay to be run immediately without additional plate coating and blocking steps
Kit Contents
The only commercially available kit for the quantitation of LONG® R3 IGF-I, the kit is fast, accurate,
and easy to use with both cell culture and drug substance samples.
The sandwich ELISA kit uses a biotinylated secondary antibody and Streptavidin-HRP for detection.
- LONG® R3 IGF-I coated strip plate, 96 wells
- LONG® R3 IGF-I Standard
- Diluent
- Biotinylated detection antibody
- Streptavidin Peroxidase
- TMB Peroxidase Substrate
- LoBind® 1.5 mL Eppendorf tubes
